Reconstitution guide
How to use your pen
Last updated: 5 October 2026
Every Optigenix pen arrives freeze-dried. The peptide is a dry powder, sealed, and stays that way until you reconstitute it with the bacteriostatic water pen supplied in the same kit.
It takes about two minutes and only has to be done once per pen. Watch the video, then follow the ten steps below. The printed guide is in the box.
- 2 minto reconstitute
- Onceper pen
- 200 units= your 2ml
In the kit
What’s in your kit
- One freeze-dried peptide pen
- One bacteriostatic water pen
- Needles
- Printed step-by-step guide
Check everything is sealed and undamaged before you start.
The ten steps
Reconstitution, step by step
-
Check your kit
Everything above should be present and sealed.
-
Remove the caps
Take the cap off the bacteriostatic water pen, then the peptide pen. Keep both caps for storage afterwards.
You should see dry powder inside the peptide pen. That is the freeze-dried peptide.
-
Attach a needle to the water pen
Screw a fresh needle onto the bacteriostatic water pen and remove the outer cover. The peptide pen does not get a needle at this stage.
Use a new needle each time. Never reuse one.
-
Fully unwind the peptide pen
Unwind the dial all the way out.
Never press the pen down — always unwind it. Pressing will expel the contents. It must stay unwound until the transfer is finished.
-
Connect the two pens
Insert the water pen needle into the top of the peptide pen. Hold both firmly and keep them straight, to avoid bending the needle or creating bubbles.
-
Transfer 200 units of water
Rotate the dial fully to 60, then press to dispense. Three full winds of 60, then one wind of 20 — 200 units, which is your 2ml. You may have a little water left in the pen; that is normal, discard it.
Do not transfer more than 200 units. Extra water dilutes the solution and the dial chart no longer applies. Go slowly and steadily. If it feels stiff, stop and check the peptide pen is fully unwound.
The peptide pen will look about two-thirds full — that is correct. It still holds the full 40mg of peptide; 2ml is used because it makes the dial chart figures come out cleanly.
-
Remove and dispose
Withdraw the water pen and dispose of it and the used needle safely in a sharps bin. Untwist the peptide pen back to zero — never press it down.
-
Swirl gently to mix
Roll the pen slowly between your palms until the solution is clear. A little may stay undissolved — leave the pen to stand until it is fully clear before you prime.
Never shake. Shaking damages peptides and creates foam.
-
Attach a needle and prime
Screw a fresh needle onto the peptide pen. Hold it upright with the needle pointing up. Dial 2 clicks and press, and repeat until a droplet appears at the tip.
Priming clears the air left in the cartridge. The small amount expelled is normal — it is about 1% of the pen. Without it, air compresses ahead of the liquid and the first dose comes up short.
-
Ready
Dial your amount, and store the pen in the fridge between 2–8°C.
Fit a fresh needle for each use, and prime with 1 click each time you do. Two clicks on first use, one click for every new needle after that.
Your dial
Your dial
Every pen we supply is a U60 — each click delivers 0.01ml. That is the same on every pen.
How many milligrams that represents depends on the pen, because each holds a different amount:
| Pen | 1 click | 5 clicks |
|---|---|---|
| Retatrutide 40mg (2ml) | 0.2 mg | 1 mg |
| GHK-Cu 100mg | 0.5 mg | 2.5 mg |
| BPC-157 / TB-500 20mg | 0.1 mg | 0.5 mg |
| CJC-1295 / Ipamorelin 10mg | 0.05 mg | 0.25 mg |
The conversion for your pen is printed on the pen label and on the card in the box. Always check it before first use — never assume a pen matches one you have used before.
Storage
Storing your pen
Before reconstitution: cool, dark and dry.
After reconstitution: refrigerate at 2–8°C.
Never freeze a pen you have already mixed. Keep it out of direct light.
Printed guide
Download the printed guide
The same instructions in a printable format, matching the guide in the box.
FAQ
Frequently asked questions
Why is the pen freeze-dried rather than pre-filled?
Peptides degrade once they are in solution — hydrolysis, oxidation and aggregation all begin immediately. A pre-filled pen has been in solution since the factory, through freight, customs and storage. Freeze-dried means the peptide stays dry and stable until you reconstitute it yourself. Read the full explanation.
How long does it last once reconstituted?
Published guidance across the research supply industry puts a reconstituted peptide in bacteriostatic water at around 28 days refrigerated. Bacteriostatic water prevents microbial growth but does not stop chemical degradation.
Why do I need to prime the pen?
There is a small amount of air in the cartridge and in each new needle. Air compresses and liquid does not, so without priming the plunger squeezes the air first and the dose comes up short. Priming clears it.
Do I have to prime every time?
Two clicks on first use, which clears the cartridge. One click each time you fit a new needle after that, which clears the needle itself.
Can I shake the pen to dissolve it faster?
No. Shaking damages peptides and creates foam. Roll it slowly between your palms.
What if the powder will not dissolve?
A little undissolved peptide is normal straight after mixing. Leave the pen to stand until it is fully clear before you prime. If it still has not cleared, contact us at info@optigenixhealth.com.
Can I reuse a needle?
No. Use a fresh needle every time and dispose of used ones in a sharps bin.
Where do I find the Certificate of Analysis for my batch?
Every batch is independently tested and published in our COA Library, searchable by batch number.
Supplied strictly for laboratory research use. Not for human consumption. Not intended to diagnose, treat, cure or prevent any disease. Purchasers must be 18 or over.